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ELISA vs LC/MS/MS details

Dairyland Analyzes Mycotoxins by Two Methods:
 
ELISA - Enzyme Linked Immunoabsorbent Assay
 
LC/MS/MS - Liquid Chromatography with two Mass Spectrometers
 
Liquid Chromatography with two Mass Spectrometers is the "gold standard" for mycotoxin analyses because it can be used for nearly any feed type and has very low detection and quantification limits.  ELISA is a faster and more economical method, with very similar detection limits to LC/MS/MS, but has not been validated for all feeds and mycotoxins.
 
Within Dairyland Laboratories, ELISA methods are used whenever validated kits are available.
 

ELISA vs LC/MS/MS Detection Limit comparison

Toxin ELISA LC/MS/MS
Aflatoxin 1.4 ppb 1.6
Vomitoxin (DON) 0.1 ppm 0.1 ppm
Zearalenone 10 ppb 20 ppb
T2/HT2 10 ppb 25 ppb
Fumonisin 0.2 ppm 0.1 ppm
Ochratoxin 1 ppb 1.1 ppb

 

LC/MS/MS also offers the ability to analyze for :Deoxynivalenol, Acetyldeoxynivalenol, Fusarenon X, Nivalenol, Neosolaniol, and Diacetoxyscirpenol.

 

Click each feed type to see which method is used for each mycotoxin. 

* Denotes USDA-GIPSA validated matrices

Alfalfa Meal

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Barley Grain

Mycotoxin Dairyland Method
Aflatoxin ELISA*
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin ELISA
All other toxins LC/MS/MS

 

Barley Flour

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Barley Silage

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Beet Pulp

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS
 

 

Canola Meal

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Grain

Mycotoxin Dairyland Method
Aflatoxin ELISA*
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin ELISA
All other toxins LC/MS/MS

 

Corn (ammoniated)

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Bran

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA*
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Cob

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Gluten Feed

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 ELISA
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Gluten Meal

Mycotoxin Dairyland Method
Aflatoxin ELISA*
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Oil

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Screenings

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Silage

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone ELISA
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Corn Starch

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Cottonseed & Cottonseed Meal

Mycotoxin Dairyland Method
Aflatoxin ELISA*
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Distillers Grain

Mycotoxin Dairyland Method
Aflatoxin ELISA*
Vomitoxin (DON) ELISA
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Distillers Syrup

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Flaxseed (Linseed)

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Hay

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Haylage

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Hominy

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Millet Grain

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Milo (Sorghum) Grain

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin ELISA
All other toxins LC/MS/MS

 

Mixed Feeds

*If every feed in a mix is validated for ELISA, then ELISA can be used. 

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Oat Grain

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin ELISA
All other toxins LC/MS/MS

 

Oat Hulls

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone ELISA
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Pellets Feeds

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

Potato

Mycotoxin Dairyland Method
Aflatoxin ELISA
Vomitoxin (DON) LC/MS/MS
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Rice Grain

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Rice Hulls

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Rye Grain

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin ELISA
All other toxins LC/MS/MS

 

Soybeans

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Soybean Meal

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Soy Flour

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Sunflower Meal

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

TMR

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) LC/MS/MS
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Wheat

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin ELISA
All other toxins LC/MS/MS

 

Wheat Bran

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA*
Zearalenone ELISA
T2/HT2 ELISA
Fumonisin ELISA
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Wheat Germ

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS

 

Wheat Middlings

Mycotoxin Dairyland Method
Aflatoxin LC/MS/MS
Vomitoxin (DON) ELISA*
Zearalenone LC/MS/MS
T2/HT2 LC/MS/MS
Fumonisin LC/MS/MS
Ochratoxin LC/MS/MS
All other toxins LC/MS/MS
For more information call Dairyland Laboratories, Inc. at 608-323-2123 or contact us here.